F thp-1 cells

Author: teqb

August undefined, 2024

WebJul 29, 2008 · However, as also observed for THP-1 cells, the percentage of KG-1 cells that expresses CD1a after differentiation is only approximately 10%, demonstrating the low DC differentiation capacity of KG-1 cells [19, 20, 23, 33]. In addition, differentiation was accompanied by distinct expression of the DC maturation marker CD83, indicating instant ... WebApr 8, 2024 · f, TMEM173 −/− THP-1 cells stably expressing STING-WT or STING-E 336 G/E 337 G (EE/GG) were infected with VACV for the indicated times and analysed by western blot.

THP-1 - TIB-202 ATCC

WebThe cell fate of THP-1-differentiated macrophages shows heterogeneity of cell growth and apoptosis. 18 Through an MTT cell proliferation assay, which can measure the reduction of tetrazolium into an insoluble formazan product in viable cells, 19 PMA-stimulated THP-1 cells significantly (p < 0.05) exhibited a decrease in the cell growth rate . WebAug 8, 2015 · THP-1 monocyte differentiation in macrophages. THP-1 cells were incubated 24 h in the presence of 150 nM PMA and then in RPMI medium during 24 h. Cells were … shares mining

Effect of Fonsecaea monophora on the Polarization of THP-1 Cells …

WebOct 7, 2024 · THP-1 cells (ATCC® TIB202™) were cultured using RPMI-1640 (Life Technologies) supplemented with 2 mM L-glutamine, 10% FCS and Penstrep (100 U/ml) at 37°C, 5% CO 2.Cells were grown to a density of 5 × 10 5 –1 × 10 6 cells/ml and used for experiments between passage 5 and 12. For THP-1 compound treatment and … WebProtocol 1. Suspend THP-1 monocytic cells at a concentration of 5 × 10 5 cells/mL in Culture media and aliquot 200 μL to each well of a flat-bottom tissue culture treated 96 … WebFeb 21, 2024 · THP-1 cells (2 × 10 5) were seeded and Staphylococcus aureus or Escherichia coli particles labeled with pHrodo red and pHrodo green were added. After 1 h of incubation at 37°C, cells were washed, stained with DAPI and analyzed using flow cytometry. THP-1 cells showed a concentration-dependent uptake of S. aureus(A & B) … shares microstrategy

THP-1 Cell Counter, THP-1 Cells Molecular Devices

Frontiers A THP-1 Cell Line-Based Exploration of …

WebFeb 22, 2024 · (F) THP-1 cells were treated as in E and analyzed by immunoblot for indicated proteins. Open and closed arrowheads indicate full-length and cleaved proteins, respectively. Movie S4. The ASC speck is highly mobile within pyroptotic cells. ASC.mCherry expressing THP-1 cells were treated with Ng and imaged every 30 … WebMay 3, 2024 · e THP-1 cells were treated with an ATPR concentration gradient (10 −5 –10 −9 M) for 72 h. Then, the protein expression of cyclin A2, CDK4, cyclin D3 and p-Rb was assessed by western blot. f THP-1 cells were treated with ATPR (10 −6 M) at different time points (0–72 h). Then, the protein expression of cyclin A2, CDK4, cyclin D3 and p ... pop is used forWebHere we present protocols that can be used to prepare M(IL-4) polarized THP-1 that transcribe CCL17, CCL26, CD200R and MRC1 and M(IL-10) cells which transcribe … shares microsoft

"WebAbstract. THP-1 is a human leukemia monocytic cell line, which has been extensively used to study monocyte/macrophage functions, mechanisms, signaling pathways, and … " - F thp-1 cells

F thp-1 cells

WebSep 1, 2024 · THP-1 cells can be differentiated into highly pure, monotypic, and well-defined DCs (TDDCs) [18]. In the present study, we successfully induced THP-1 cells to …

Did you know?

Although THP-1 cells are of the same lineage, mutations can cause differences as the progeny proliferates. In general, THP-1 cells exhibit a large, round, single-cell morphology. The cells were derived from the peripheral blood of a 1-year-old human male with acute monocytic leukemia. Some of their characteristics are: • Expression of Fc receptor and C3b receptors while lacking surface and cytoplasmic immunoglob… WebPanel A) Light microscopy photographs of cultured THP-1 monocytes and macrophages. Upper panel A.1 show the normal monocytic cell phenotycpic (round cells grown in …

WebRemove medium and resuspend the cell pellet at a density of 3 - 5 x 100,000 cells/ml in fresh medium containing 20% serum. Incubate flask at 37°C; 5 - 7% CO2. Check daily. Keep flask in a vertical position until the cells reach the exponential phase of growth. This can take up to 7 days. WebApr 10, 2024 · F. monophora promoted the polarization of THP-1 cells to classically activated macrophages and inhibited that of THP-1 cells to alternatively activated macrophages. Chromoblastomycosis is a chronic, progressive fungal disease of the skin and subcutaneous tissue caused by a unique group of dematiaceous fungi.

WebGrow THP-1 cells in these flasks until a concentration of approx.. 1x106 cells/ml. · Prepare freezing medium and cool down on ice at least one hour before cell harvesting. Freezing medium. · 60% ... WebNov 16, 2024 · THP-1 cells (ATCC TIB-202) were cultured in RPMI1640 [final concentrations: 4.5 g/L glucose, 2 mM L-GlutaMAX TM, 10 mM HEPES and 1 mM sodium pyruvate] containing 10% (v/v) FBS and 0.05 mM 2-mercaptoethanol. THP-1 monocytes were terminally differentiated using 100 nM phorbol 12-myristate 13-acetate (PMA) for 24 h.

WebJan 18, 2024 · 鲍曼不动杆菌外膜囊泡诱发人单核-巨噬细胞THP-1的炎性反应. 1.扬州市第三人民医院，江苏扬州 225000； 2. 江苏大学医学院，江苏镇江 212013. 鲍曼不动杆菌 (Acinetobacterbaumannii,A.baumannii)是目前临床常见病原菌，近年来各类抗生素的长期大量使用，鲍曼不动杆菌 ...

WebAug 10, 2024 · The anti-inflammatory effects and potential mechanisms were investigated in LPS-stimulated THP-1 cells, using salvianolic acid B (SalB) as the positive control. The enzyme-linked immunosorbent assays (ELISA) were used to determine the secretory protein levels of interleukin-1β (IL-1β), interleukin-6 (IL-6) and tumor necrosis factor-α (TNF-α ... shares modWebApr 6, 2024 · Cell Counting Kit-8 (CCK-8) (Beyotime, China) was used to measure cell proliferation. Briefly, the cells were separately seeded on the surface of the meshes (1 cm × 1 cm) with a density of 100,000 cells/cm 2, and were cultured in medium (THP-1 cells need 100 ng/mL of PMA) for 24 h and 48 h. After treatment, 20 μL of CCK-8 solution was … pop it adjustable sliding cabinetWebTHP-1 cells were treated with the VCC toxin at 10, 15, 30 and 60 min. LPS (1 µg/mL) was used as a positive control. The VCC starts the transcriptional regulation of subunits of NF-κB, p50. The mRNA expressions were evaluated using qp-PCR. Changes in expression were compared with the control without treatment and normalized with the ... pop it 256 bubblesWebWe incubated unprimed THP-1 cells either in high K + containing media or pre-treated these cells with broad-spectrum chloride channel inhibitor NPPB (50 μM), or NBC19: a … shares molecular partnersWebJan 12, 2024 · Mastering the Art of Growing THP-1 cells. Published January 12, 2024. Posted in: Cells and Model Organisms. Tissue culture can sometimes seem like a black art. Too careful—your cells go down. Not … share smith optics forefront helmetWeb3.1.2.1 Labeling effector cells and target cells. 1. Count THP-1 cells. If cell number allows, duplicate or triplicate wells are set up for each condition. 2. Count the target cells. Take … share smr incWebMar 3, 2024 · Briefly, for migration assay, 200 μL of cell suspensions without FBS (for THP-1 cells, 1 × 10 5 cells pretreated with 100 ng/mL PMA (Sigma-Aldrich, P8139) for 24 hours and rested in RPMI 1640 with 10% FBS for another 24 hours; for KFs and NFs, 3 × 10 4 cells pretreated with 500 nM AS1517499 (or DMSO control) were added to the upper 24 … shares motley fool