Determination of aconitase activity

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August undefined, 2024

WebJun 1, 2015 · The effect of pH on aconitase activity was studied in 100 mM tris–HCl buffer containing 40 mM citrate or 4 mM d, l-isocitrate. Determination of K i was performed by plotting the graphs of dependence of 1/v from the concentration of inhibitor [i] at two fixed concentrations of substrate (citrate) (Dixon, 1953). WebDetermination of Aconitase Activity 4.5 ml. of water in each of a series of thick walled 100 X 14 mm. Pyrex test-tubes are brought to 30”.

FACTORS AFFECTING THE ACTIVITY OF ACONITASE - ScienceDirect

WebAug 1, 2005 · Unanticipated, however, was the reduced expression of the tricarboxylic acid (TCA) cycle gene citB (encoding aconitase), resulting in the loss of aconitase activity and preventing the ... WebSep 11, 2014 · In the aconitase activity assay protocol, aconitase catalyzes an equilibrium between aconitate, cis-aconitate and iso-citrate. These reactions are monitored by … black adam poster imp awards

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WebTotal aconitase activity can also be measured in whole cell lysates, for example, 143B osteosarcoma lysate shown below (Figure 3). Note – the aconitase activity measured in … WebAug 29, 2024 · First, in-gel activity assays showed that the mitochondrial aconitase activity (m-aco) in FRDA patient-derived cells increased significantly after SS-31 treatment. ... Determination of ... black adidas athletic shoes

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WebFor quantitative determination of aconitase enzyme activity and high-throughput screening assays for aconitase modulator. Key Features. Fast and sensitive. Linear detection range (20 µL sample): 0.5 to 100 U/L for 20 min reaction. Convenient and high-throughput. Homogeneous “mix-incubate-measure” type assay. WebJul 9, 2004 · Aconitase, a Krebs-cycle enzyme that converts citrate to isocitrate, belongs to the family of iron-sulfur–containing dehydratases whose activities depend on an intact cubane [4Fe-4S] 2+ cluster (1, 2).The purified enzyme is highly susceptible to oxidant-induced inactivation due to release of the solvent-exposed Fe-α and formation of a [3Fe … black aio coolerWebDetermination of aconitase activity Cell pellets of the bioreactor samples were washed once with 10 ml washing buffer (20 mM Tris/HCl, pH 8.0) and then resuspended in 4 ml ice-cold lysis buffer (20 mM Tris/HCl, pH 8.0, 5 mM dithio-threitol). Mycelium was broken down by ultrasonic treatment in an black and brown bathroom rugs

"WebSomething that is curious about aconitase is the fact that it uses iron as the inorganic substituent. Mutanogenic studies on aconitase have found substituting the iron ions with … " - Determination of aconitase activity

Determination of aconitase activity

Quantitative relationship between inhibition of respiratory …

WebAconitase activity determined after 15 hours incubation at 5', pH 7.4. effect. Phosphate thus competed with the protein for the iron in the system, as well as prevented the added … WebJan 10, 2024 · This protocol describes the determination of aconitase activity, which can provide an indirect measurement of Lon activity in vivo. The protocol is optimized for …

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WebMitochondrial aconitase is a reversible enzyme that catalyzes the conversion of citrate to isocitrate in the tricarboxylic acid cycle. Mitochondrial aconitase is very sensitive to oxidative... Determination of Aconitase Activity: A Substrate of the Mitochondrial Lon Protease Springer for Research & Development WebJan 1, 2024 · Determination of Aconitase Activity: A Substrate of the Mitochondrial Lon Protease. ...

WebOct 14, 1997 · The aconitase activity was determined in the supernatant using two different methods. Method 1 is a coupled assay in which the formation of NADPH was … WebDetermination of Aconitase Activity—For the determination of the aconitase activity, cells from the CGXII main culture (∼25 ml) were harvested with ∼25 g of crushed ice …

WebAconitase 2 ( ACO2 ) encodes the mitochondrial aconitase (ACO2), an enzyme catalyzing interconversion of citrate into isocitrate in the Krebs cycle. ACO2 mutations have been initially associated with infantile cerebellar-retinal degeneration combining optic atrophy, retinal degeneration, severe encephalopathy, epilepsy, and cerebellar ataxia1–3; … WebAug 31, 1999 · The aconitase protein of Bacillus subtilis was able to bind specifically to sequences resembling the iron response elements (IREs) found in eukaryotic mRNAs. The sequences bound include the rabbit ferritin IRE and IRE-like sequences in the B. subtilis operons that encode the major cytochrome oxidase and an iron uptake system. IRE …

WebSep 17, 2024 · These intermediates are capable of oxidizing the cluster, which leads to iron release and consequent loss of the catalytic activity of aconitase. As the reaction of the Fe-S cluster with O 2 •-is fast (∼10 7 M-1 s-1), quite specific, and reversible in vivo, quantification of active aconitase has been used to evaluate O 2 •-formation in cells.

WebApr 14, 2024 · Plants of the genus Scutellaria (Lamiaceae) have a wide variety of bioactive secondary metabolites with diverse biological properties, e.g., anti-inflammatory, antiallergenic, antioxidant, antiviral, and antitumor activities. The chemical composition of the hydroethanolic extracts, obtained from dried plants of S. incarnata, S. coccinea, and … black and copper lightWebJul 1, 2007 · We examined the hydroxyketone-mediated production of reactive oxygen species by determination of the aconitase activity. Aconitase is the most sensitive enzyme to reactive oxygen species, and thus, we can estimate the generation of superoxide radical by the inactivation of the enzyme (Gardner and Fridovich, 1992, Murakami and … black american short haired catsWebDec 10, 2024 · Mitochondrial Aconitase In-Gel Enzyme Activity Assays Protein samples were separated using a non-denaturing gel, and then the gel was incubated in a coloring … black and decker waste binWebThus, aconitase activity is used as an indicator of levels of intracellular H 2 O 2 and its oxidative damage to cellular components. For example, substantially decreased … black and gold bathroom wall sconcesWeb0.5M sorbitol at the concentration of 200mg/ml. Aconitase activity was determined by increase in absorbance at 340nm in the presence of 5mM citrate, 0.25mM NADP, 4mM MgCl 2, 10unit/ml of NADP-isocitrate dehydroge-nase (Murakami et al., 2006). 2.3. Determination of hydrogen peroxide and superoxide anion Determination of rosmarinic … black and french lublinWebBeef heart aconitase has been studied by low temper- ature magnetic circular dichroism (MCD) spectroscopy in the wavelength region 300 to I900 nm. Together with parallel electron paramagnetic resonance and ac- tivity measurements, these data enable correlations between Fe-S cluster-type and enzymic activity in aconitase. black and gold synthetic hairWebThe aconitase activity was significantly depressed in the 2% O2 group (*P ⬍ 0.03, n ⫽ 6/group). ... Determination of the mechanism of free radical generation in damage in the presence of cyanosis: a new concept of controlled cardiac human aortic endothelial cells exposed to anoxia and reoxygenation. ... black and gold old fashioned glasses