Cntf mrna
WebMay 19, 2024 · (A) The knockout of Cntf was confirmed by examining the mRNA level of Cntf in DRG harvested from Cntf +/+ and Cntf −/− mice. n = 6. (B) CNTF protein in sciatic nerve homogenate was measured by ELISA. n = 4. (C) CNTF and S100 were determined by immunohistochemistry on sagittal sciatic nerve sections harvested from Cntf +/+ and … WebThe mRNA for the alpha chain specific for the CNTF receptor was detected by Northern blot in tumors secreting prolactin (PRL) and GH and in non-functioning tumors. We found that both IL-11 and CNTF exerted a similar stimulatory effect on GH mRNA expression in somatotropic monolayer cell cultures from acromegalic tumors, but these cytokines had ...
Cntf mrna
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WebMar 21, 2024 · This gene represents a read-through transcript composed of ZFP91 and CNTF sequence. This transcript is thought to be non-coding because it would be subject to nonsense-mediated mRNA decay (NMD). Read-through transcription of ZFP91 and CNTF has also been observed in mouse. [provided by RefSeq, Aug 2008] WebRGCs were immunoreactive for CNTF and quantitative RT-PCR revealed a substantial increase in CNTF mRNA expression in retinas transduced with AAV-CNTF-GFP. The …
WebJul 27, 2011 · CNTF has been detected in astrocytes in the remyelinating phase after viral-induced spinal cord demyelination . In contrast, in the lysolecithin model CNTF mRNA expression was not up-regulated during remyelination . The conflicting results of CNTF expression in different demyelination models and CNS regions may be due to the … Multiple sclerosis (MS) affects more than 1.5 million people worldwide and is the major cause of non-traumatic neurological disability in young adults in North America and Europe (Weinshenker, 1996; Noseworthy et al., 2000). Approximately 85% of MS patients initially have a relapsing-remitting disease course (RR … See more This work was supported by the NIH (NINDS) grants RO1 NS35058 and PO1 NS38667 (B.D.T). Authors would like to thank Thalia Torres for her excellent technical help with … See more Myelinated, non-lesion motor cortex samples from six MS patients and six individuals without neurological disease were obtained at autopsy and compared on Affymetrix U133A … See more Neurodegeneration is a major cause of permanent neurological disability in chronic MS patients. Remyelination, redistribution of … See more
WebNov 24, 2015 · Additionally, by quantitative PCR with reverse transcription to measure the Cntf mRNA expression in the cervical spinal cord (C4–C7) after pyramidotomy, we found that compared with the sham control, the CNTF mRNA levels were significantly increased at 1-day post injury (Fig. 2b), suggesting that the CNTF upregulation is likely due to the ... WebMay 18, 2006 · To determine the amount of CNTF mRNA present in transduced eyes, retinas from four rats that did not undergo ON injury …
WebNov 4, 2013 · CNTF interacts with a tripartite receptor complex consisting of two single-pass membrane spanning receptor subunits (gp130 and LIFRβ) and an additional ligand …
WebMay 18, 2006 · To determine the amount of CNTF mRNA present in transduced eyes, retinas from four rats that did not undergo ON injury were removed several weeks following eye injections of AAV-CNTF-GFP … pyvista point cloud to meshWebQuantitative real-time PCR analysis detected a high level of Cntf mRNA, which was transiently upregulated post-SNI in the sciatic nerves (Figure S1I). The CNTF protein was abundant in the sciatic nerve tissue lysates, with a concentration >200 ng/mg detected by ELISA (Figure 3B). pyvista save_meshioWebNov 24, 2015 · (b) Cntf mRNA measurement by quantitative PCR with reverse transcription of the spinal cord at 1 and 3 days post injury, respectively. Cntf mRNA expression levels were normalized to Gapdh mRNA levels. pyvista save point cloudWebOct 15, 1991 · The olfactory bulb of adult rats has comparably high levels of CNTF to the optic nerve, and here again, CNTF-immunoreactivity is localized in a subpopulation of … pyvista siluetteWebCiliary neurotrophic factor. Ciliary neurotrophic factor is a protein that in humans is encoded by the CNTF gene. [5] [6] [7] The protein encoded by this gene is a polypeptide hormone and neurotrophic factor whose actions have mainly been studied in the nervous system where it promotes neurotransmitter synthesis and neurite outgrowth in certain ... pyvista slice along lineWebData indicate that aspirin increased mRNA and protein expression of CNTF in primary mouse and human astrocytes in a dose- and time-dependent manner. we show that the … pyvista showWebMay 23, 2007 · At a higher magnification, CNTF mRNA was evenly distributed throughout the cytoplasm in the neurons (arrows in Fig. 5b). The positive reactions were suggested to be specific for CNTF mRNA because the sense probe for CNTF failed to hybridize in the cells (Fig. 5d). These findings suggest that CNTF protein in cultured DRG neurons is, at … pyvista spline